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. 2001 Nov;127(3):1113–1124.

Figure 3.

Figure 3

Construction of the Synechocystis sp. PCC 6803 SLR1736::Kmr mutant. A, Simplified scheme of the wild-type SLR1736 ORF in the Synechocystis sp. PCC 6803 genome. Insertion of a kanamycin (Kmr) cassette into MfeI site of the SLR1736 ORF and the SLR1736F and R PCR primers (F and R, small arrows) are indicated. B, Autoradiograph of the PCR products amplified from wild type (lane 1) and the SLR1736::Kmr mutant (lane 2) genomic DNA. No wild-type copies of SLR1736 were detected in the SLR1736::Kmr mutant.