Figure 3.

Organ-specific expression pattern of tomato LeXET2. Poly(A⁺) RNA was isolated from different vegetative tissues (a) and tomato pericarp (b) at different stages of fruit development and was analyzed by northern blot (1 μg lane⁻¹). Blots were hybridized with a LeEXT2 cDNA probe and washed in 0.5× SSC at 65°C. The fruit blot was subsequently hybridized with a tomato actin probe as a loading control. Exposure to photographic film was 30 times longer for the fruit tissue blot than for the vegetative tissue blot. I, 0.5- to 1-cm diameter fruit; III, 4- to 6-cm diameter fruit; IG, immature green; MG, mature green; Br, breaker; LR, light red.