Figure 3.

Multiple alignments of known MS amino acid sequences from several fungi that were used for designing PCR primers and for comparison with the deduced full-length sequence for MS from G. intraradices. Sequences are shown for Hansenula polymorpha, Candida tropicalis, E. nidulans, N. crassa, S. cerevisiae, and G. intraradices. The degree of homology among the different sequences at each residue is indicated as complete conservation (*), high homology/conserved substitutions (:), moderate conservation (.), or little or no homology (unmarked). Shown in bold are the C-terminal tripeptide glyoxysomal-targeting sequences Ser-Lys-Leu and Ala-Lys-Leu that are present in G. intraradices and other fungal sequences.