Fig. 5.

ABE9-SpRY can effectively produce Tpc1^I486T and Trpm4^L903P founder mice. A-to-G editing frequencies plotted across each adenine position are shown in (a) for 48 Tpc1^I486 adult mouse biopsies, and (d) for 36 Trpm4^L903 adult mouse biopsies. The frequency of desired editing (without bystander edits) and indels are presented in (b) for Tpc1^I486 adults, and (e) for Trpm4^L903 adult mice. Product purity is calculated as the percentage of correctly edited sequences relative to the total number of modified alleles and is displayed in (c) for Tpc1^I486 adults, and (f) for Trpm4^L903 adult mice. Data are presented as standard boxplots (white) following the Tukey convention, which includes the median (black horizontal line), displayed within violin plots (red), and individual data points (black) representing each biological replicate. Crossing schemes of F0 founder mice, with numerical data for F1 and F2 transmission for Tpc1^I486T (g), and Trpm4^L903P (h), respectively. Asterisk note (b, e): The value shown beneath “perfect” indicates the number of F0 founders classified as high-confidence clean allele-carrying at the target locus, using read-level allele classes within the CRISPResso2 quantification window. High-confidence founders were defined as those with ≥20% desired-only (“perfect”) reads and <2.5% unwanted outcomes(unwanted = "desired + bystander" + bystander-only + indels). Full per-founder allele-class compositions and the classification are provided in Supplementary Fig. S9.