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The effect of external pH on KH channel gating. GK-EM relationships (mean ± se) of A, four extensors, and B, five flexors at [K+]O of 200 mm at the indicated pHs. Lines are Boltzmann functions fitted to the averaged (GK- EM) data, with the best fit parameters listed in Table II (see “Materials and Methods” for details of averaging). Arrows indicate E1/2 values. Note the progressive shift of E1/2 to more negative values. Solutions: bath: (c) or (d)/pipette: (f).
