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. 2005 Nov 27;33(20):e179. doi: 10.1093/nar/gni178

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Comparison of heat-treated cells, cell lysate and total RNA for real-time quantitation of 10 miRNAs. The level of miRNA expression is measured in the threshold cycles (C_T). Approximately 400 HepG2 cells were analyzed per PCR.