Table 2.
Discrimination between mature miRNAs and their pri- or pre-miRNA precursors
| ID | Synthetic miRNA (No. of copies) | Synthetic precursor (No. of copies) | Total RNA (ng) | CT miRNA | Precursor |
|---|---|---|---|---|---|
| miR-26b | 1.5 × 108 | 0 | 0 | 16.5 | ND |
| 0 | 1.5 × 108 | 0 | 27.4 | 18.7 | |
| 0 | 0 | 7.5 | 21.9 | 28.7 | |
| 0 | 0 | 0 | ND | ND | |
| let-7a | 1.5 × 108 | 0 | 0 | 16.5 | ND |
| 0 | 1.5 × 108 | 0 | 29.5 | 19.4 | |
| 0 | 0 | 7.5 | 19.9 | 34.7 | |
| 0 | 0 | 0 | ND | ND | |
| miR-30a-3p | 1.5 × 108 | 0 | 0 | 15.8 | ND |
| 0 | 1.5 × 108 | 0 | 24.2 | 17.2 | |
| 0 | 0 | 7.5 | 25.4 | 30.3 | |
| 0 | 0 | 0 | ND | ND | |
| miR-30a-5p | 1.5 × 108 | 0 | 0 | 16.3 | ND |
| 0 | 1.5 × 108 | 0 | 29.1 | 18.9 | |
| 0 | 0 | 7.5 | 22.1 | 30.3 | |
| 0 | 0 | 0 | 39.6 | ND |
Note: ND represents no detectable PCR products after 40 cycles. The copy number of synthetic miRNAs in RT was estimated based on the A260 values. Only 9% of RT product was added to PCR. Total RNA from human lung was used. Pre-miRNA precursors, pri-let-7a-3 and pri-miR-26b, and pre-miRNA precursor pre-miR-30a were examined.