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. 2026 Feb 20;26:75. doi: 10.1186/s12906-026-05277-7

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© The Author(s) 2026

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — CH seed promotes DAF-16 nuclear localization. a Cytoplasmic localization. b Intermediate localization. c Nuclear localization. Scale bar, 20 μm. d Data showing the percentage of worms with nuclear localization. CH (100 µg/ml) and CH (200 µg/ml) increased the translocation of DAF-16::GFP in TJ356 mutant worms to the nucleus. The data are shown as the means ± SEMs for four different experiments, 20–30 worms/group, and were analyzed via one-way ANOVA followed by the Bonferroni correction (post-hoc). *p < 0.05 and **p < 0.01 compared with the solvent control group