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. 2002 Aug 20;99(18):11652–11657. doi: 10.1073/pnas.172382799

Figure 6.

Figure 6

The overexpression of the C2A domain of JFC1 does not disrupt actin polymerization. NIH 3T3 cells were transfected with the EGFP-C2A construct, and polymerized actin was visualized by using rhodamine-labeled phalloidin. (Left) Fluorescence image of the C2A domain of JFC1. (Right) Fluorescence image of phalloidin-rhodamine. The less apparent membrane staining of JFC1-C2A observed in this figure was caused by the fixation process. This appearance has been described for other membrane-binding proteins (25) and does not affect the validation of the result.