GATA-1 regulates pol II recruitment to the adult β-globin promoters and the LCR. ChIP analysis of GATA-1, p45/NF-E2, and pol II binding to the β-globin locus in G1E and G1E-ER-GATA cells. (Upper) Graphs depict the linearity of input signals. (Lower) Signals obtained for immunoprecipitated samples are graphed and are expressed relative to the 0.06% input. The graphs show the relative intensity of PCR products from at least three independent experiments (mean ± SEM). IgG, rabbit IgG; Rat, rat IgG; PI, rabbit preimmune sera.