Figure 4.

Quantitative analysis of GATA-1-dependent pol II and NF-E2 recruitment. Samples from three independent ChIP experiments that were included in the analysis of Fig. 3 were analyzed by real-time PCR. (Upper) The linearity of input signals. (Lower) The signals obtained for immunoprecipitated samples are graphed and are expressed relative to a standard curve generated from input samples (mean ± SEM). Values shown above the bars represent the ratio of signals obtained relative to the appropriate control antibody. Variations in background signals alter the ratios, although the variations did not exceed 2-fold. Rat, rat IgG; PI, rabbit preimmune sera; NC, no chromatin.