Figure 1. Aneuploidy renders cells resistant to oxidative stress.

(A) Acute and chronic aneuploidy models. (B) DNA content in diploid and aneuploid hCECs. (C) Viability of treated diploid and aneuploid hCECs (TP53WT). (D) Representative images of H₂O₂-treated cells (75 μM, 24h). (E) Viability quantification from (D). (F) Arm-level somatic copy number alterations in hCEC clones (TP53KO). (G) Viability of H₂O₂-treated hCEC clones (50 μM H₂O₂ for 1h + 24h release). (H) Viability of H₂O₂-treated HCT116 or RPE1 clones (4mM and 100 μM, respectively, 24h). (I) Viability (top left) and representative images (right) of H₂O₂-treated KP cells (2 mM, 24h). Proliferation curves (bottom left). (J) Representative images and viability of H₂O₂-treated colon cancer cells (4 mM, 24 h). One-way ANOVA with Dunnett’s test (C, D, HCT116 in H). Tukey’s test (G, J). unpaired t-test (RPE1 in H, I). Scale bar, 50 μm.