Figure 2. PARP1 is suppressed in aneuploid cells and required for ROS-induced cell death.

(A) DNA damage in diploid and aneuploid hCECs after H₂O₂ (50 μM, 1h). Left: representative images. Right: quantification of damaged DNA. Medians with 95% CI. (B) Representative images of H₂O₂-treated hCECs (50 μM, 1h) with cell death inhibitors (2h pretreatment + 24h post-treatment). (C) Viability quantification from (B). (D) Relative PARP1 RNA levels (Log2) in acute aneuploidy. (E) PARP1 protein levels in acute aneuploidy. (F-G) Correlation between aneuploidy degree and PARP1 RNA (F) or protein (G) levels in hCEC clones. (H) PARP1 protein levels in hCEC clones. (I) Relative PARP1 protein levels in HCT116 and RPE1 clones (data from Stingele et al., 2012). Notation: cell line-chromosome-copy number Kruskal-Wallis one-way ANOVA with Dunnett’s test (A). One-way ANOVA with Dunnett’s test (C, hCEC in D); Tukey’s test (H). Unpaired t-test (RPE1, TP53KO RPE1, and HCT116 in D). One sample t-test against zero (I). Scale bar, 50 μm.