Extended Data Figure 15. Charge-neutralizing mutagenesis in the frequently deleted region turns ASXL1 leukemogenic.

(a) Venn diagrams showing the overlap between the genes that were more highly expressed (fold change > 2, probability > 0.9) by transduction of ASXL1 (1–1067) 36EQ (salmon) than 1–1067 and genes that were more highly expressed by ASXL1 (1–1067) 34DN (cyan) than by 1–1067. P values by two-sided Fisher’s exact test.

(b) Venn diagrams for the genes that were expressed more highly (fold change > 2, probability > 0.9) by transduction of ASXL1 (1–1067) 36EQ than by 1–1067, and their overlap between RNA-seq assays before (salmon) and after (cyan) colony formation as shown in Fig. 5b.

(c) Heatmap for the relative expression levels for the overlapped genes in (b).

(d) Significantly enriched Biological Processes for the overlapped genes in (b). By Metascape. P values by two-sided hypergeometric test.

(e) Venn diagrams showing the overlap between the genes (salmon) that were expressed more highly (fold change > 2, probability > 0.9) by transduction of ASXL1 (1–1067) 36EQ or 34DN than by ASXL1 (1–1067), from RNA-seq before colony formation, and the genes (cyan) that were induced less efficiently by ASXL1 (1–590) 10RA than 1–590, from RNA-seq in Fig 3a. P values by two-sided Fisher’s exact test.