Table 3.
Cytokine production by CD4+ T cells after short-term Th1/Th2 differentiation in vitro
| Primary stimulus | IFN-γ, pg/ml
|
IL-4, pg/ml
|
TNF, pg/ml
|
IL-5, pg/ml
|
||||
|---|---|---|---|---|---|---|---|---|
| IRF4+/− | IRF4−/− | IRF4+/− | IRF4−/− | IRF4+/− | IRF4−/− | IRF4+/− | IRF4−/− | |
| Anti-CD3 | 4,000 | <40 | 16 | <2 | <4 | <4 | 20 | <5 |
| Anti-CD3, anti-IL-4, IL-12 | 300,000 | 12,000 | 2 | <2 | 550 | <4 | <5 | <5 |
| Anti-CD3, IL-4, anti-IL-12 | 50,000 | 500,000 | 50,000 | 150 | 180 | 1,500 | 18,000 | <5 |
CD62L+CD4+ T cells from IRF4+/− and IRF4−/− mice purified by magnetic cell sorting were stimulated with immobilized anti-CD3 (5 μg/ml) and/or cytokines for 96 h, as indicated. Thereafter, they were rested for 72 h in the presence of IL-2 and restimulated in triplicate cultures with anti-CD3. Twenty-four-hour culture supernatants were tested for cytokines by ELISA. Data shown give the mean of the triplicates; the SD was in all cases <10%. Results shown are representative of six (IFN-γ, IL-4) and three (TNF, IL-5) different experiments. ND, not determined.