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Pattern of NY-ESO-1-tetramer staining of CD8+ T cells in a vaccinated patient with preexisting spontaneous NY-ESO-1 immunity. CD8+ T cells from patient NW415 were presensitized with S9L (Left), S11L (Center), or L9L (Right) and stained at day 9 with tetrameric complexes of HLA-A2 molecules and S9C (Top), S11L (Middle), or L9L (Bottom) peptide, along with anti-CD62L. Lymphocytes gated for CD8+ expression are shown.
