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Persistence of the CD8+ T cell response after a single immunization with sporozoites. Normal mice received transgenic (Tg) CD8+ T cells; 24 h later, they were immunized (⧫) i.v. with 5 × 104 attenuated sporozoites or were not immunized (■). The frequencies of epitope-specific CD8+ T cells in the spleen (Upper) and the liver (Lower) were determined by ELISPOT. Results represent one of two similar experiments expressed as mean values + SD.
