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AMPK activity and phosphorylation state in mouse adipose tissue and liver. (Upper) Results of triplicate determinations (mean ± SEM) of AMPK activity, measured as in Materials and Methods, in the presence of saturating concentrations of AMP (200 μM). The data are expressed as pmol P incorporated per minute per milligram total protein immunoprecipiates (in triplicate), blotted with an antibody specific for Thr-172, the activating phosphorylation site of AMPKα.
