Table 5.
Real-time PCR analysis for abundance of AAV vectors in nu/nu mouse liver after injection of 1 × 1011 genome copies of vector
| AAV vectors/dose | Genome copies per cell |
|---|---|
| AAV2/1AlbA1AT | 0.6 ± 0.36 |
| AAV2AlbA1AT | 0.003 ± 0.001 |
| AAV2/5AlbA1AT | 0.83 ± 0.64 |
| AAV2/7AlbA1AT | 2.2 ± 1.7 |
| AAV2/8AlbA1AT | 18 ± 11 |
A set of probe and primers targeting the SV40 poly(A) region of the vector genome was used for TaqMan PCR. Values shown are means of three individual animals with standard deviations. The animals were sacrificed at day 56 to harvest liver tissues for DNA extraction.