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. 2002 Aug 23;99(18):11878–11883. doi: 10.1073/pnas.182552499

Figure 7.

Figure 7

EBNA2 mediates cell survival by inhibiting Nur77 nuclear export. Immunofluorescence assays examining GFP-Nur77 localization in cells expressing EBNA2 and subjected to apoptotic stimuli. (A) HeLa cells were cotransfected with GFP-Nur77 and either E2wt or E2Δ123–147 and treated with PMA (200 ng/ml for 16 hr). (B) GFP-Nur77-transfected NIH 3T3 cells were infected with SV-E2wt (Upper) or SV-E2Δ123–147 (Lower) and fixed 24 hr after infection. (C) Flag-NotchIC- and HA-Nur77-transfected HeLa cells treated as in A. Nur77 (green) remains nuclear in E2wt- (red) and Flag-NotchIC-expressing (red) cells. Colocalized proteins appear yellow in the merged images. Nuclei were stained with DAPI (blue).