Figure 2.

Androgen-regulated expression of characterized and previously uncharacterized genes. (A) Northern analysis confirmation of ARG expression in LNCaP prostate cancer cells treated with the synthetic androgen R1881 (+) or vehicle control (−) for 24 h. For each gene, the corresponding microarray-derived fold change in expression is provided below the gene name. (B) Hierarchical cluster analysis of uncharacterized genes exhibiting a temporal expression profile corresponding to PSA. *, genes to the left of the vertical bar represent characterized ARGs with tissue-expression profiles enhanced or restricted to the prostate. (C) Northern analysis confirmation of uncharacterized ARGs in LNCaP cells treated with androgen (+) or vehicle control (−) for 24 h. Multiple tissue Northern blot of selected uncharacterized ARGs demonstrating prostate-restricted or prostate-enhanced expression relative to other normal human tissues. §, one alternative spliced form of Hs.288821 exhibits prostate-enhanced expression.