Figure 4.

Abrogation of restriction of SIV-eGFP by SIV-LacZ and HIV-puro in African green monkey CV1 cells. TE671 (□), LLC-MK2 (●), OMK (×), or CV1 cells (▴) were exposed to 5 × 10⁶ TE671 i.u. of SIV-LacZ or 5 × 10⁶ TE671 i.u HIV-puro (♦) (CV1 only) for 4 h, washed, and exposed to 5 × 10⁴ i.u. of SIV-eGFP. Percentage infection was measured by analysis of eGFP expression 48 h later by FACS. Fold abrogation is calculated by dividing the increased percentage infection after abrogation by unabrogated control HIV-eGFP infection. Approximately 1% of unabrogated monkey controls and 10% of human TE671 controls typically were infected. Data are representative of two independent experiments.