Figure 3.

Salvinorin A is a potent KOR agonist. A shows that Salvinorin A potently inhibits 3H-bremazocine binding to cloned KORs, whereas B shows the ability of Salvinorin A to inhibit forskolin-stimulated adenylate cyclase in KOR-393 cells. Data represent the mean ± SD of triplicate determinations from a representative experiment that has been replicated three times. For the inhibition of forskolin-stimulated cyclase activity, an EC₅₀ value of 1 ± 0.5 nM was calculated for Salvinorin A, compared with an EC₅₀ value of 1.2 ± 0.6 nM for U69593.