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. 2002 Aug 14;99(18):11946–11950. doi: 10.1073/pnas.182296499

Figure 1.

Figure 1

VEGF stimulates BrdUrd incorporation in murine cortical cultures in vitro. (A) Immunodetection of BrdUrd labeling (red) in control (Left), and 10 ng/ml VEGF-treated (Right) cortical cultures at 24 h in vitro. (Original magnification, 20×; Insets, 40×.) (B) Quantification of VEGF-stimulated BrdUrd incorporation by ELISA, expressed as a percentage of BrdUrd incorporation in control cultures. (C) Cell counts (number of DAPI-stained nuclei) in VEGF-treated cultures, expressed as a percentage of cell counts in control cultures. (D) Colocalization of BrdUrd labeling (red) with immunoreactivity for the immature neuronal marker ENCAM (green, Left) and the neuroepithelial precursor cell marker nestin (green, Right). Data are representative fields (A and D) or mean values ± SEM (B and C) from at least three experiments per panel.