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HOCl-mediated LDH leakage: Effect of NO
. NO
was added to cells in EBSS 10 min before HOCl (▪) or NO
premixed with HOCl (200 μM) for 5 min and then added to cells for 10 min (□). After treatment cells were washed in DMEM, and LDH leakage was measured in the medium after 24 hrs as described in Experimental Procedures. Data are expressed as mean ± standard deviation of six or more separate experiments. *, P < 0.1; **, P < 0.05; and ***, P < 0.01 compared with HOCl addition alone.
