Fig 3.

Cleavage of N-terminal fragments of hTop3α(1–1001) fused to EGFP on mitochondrial import. Whole-cell lysates from HeLa cells transfected with constructs expressing EGFP, hTop3α (1–34)-EGFP, hTop3α (26–63)-EGFP, and hTop3α (1–63)-EGFP (lanes 3–6) were analyzed by electrophoresis in a SDS-polyacrylamide gel, and probed with anti-GFP antibodies. Lanes 1, 2, and 7 contained molecular-mass standards; the known molecular masses of the bands (in kilodaltons) are indicated in the left-hand margin. Lane 8 contained a control lysate from untransfected HeLa cells.