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Immunogold labeling of spliceosomes. Spliceosomes assembled on AdML-M3 pre-mRNA were incubated with antitrimethyl cap antibody followed by a secondary antibody conjugated with 10 nm gold (A) or with anti-U5–40kD antibody followed by a secondary antibody conjugated with 6 nm gold (B). Arrows indicate the positions of gold labels. The 3-cm bars are equal to 180 nm (A) and 110 nm (B), respectively.
