Fig 6.

Drosophila RHO-1 can effectively rescue the cell communication phenotype associated with aarA deletion in P. stuartii. Namely, RHO-1 expression rescues the mutant's ability to produce an extracellular activating signal. Conditioned medium from an aarA deletion strain (XD37.A) containing an empty vector activates sensor expression to a level comparable to LB medium alone (27 ± 2 and 23 ± 1 units, respectively). In contrast, medium conditioned by XD37.A/pBCSK+Rho-1 activated the sensor to a level 24-fold above medium alone and 20-fold above that seen for XD37.A/pBCSK (541 ± 59 units). Medium conditioned by XD37.A/pAARA activated the sensor to similar levels (490 ± 6 units).