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Mechanism of RHO and AarA action. (a) RHO acts in the fly as an I-CliP to release the luminal portion of SPI. (b) In analogy, and in a similar manner to the release of the cAD1 in E. faecalis, we propose that AarA works as an I-CliP to release a peptide pheromone in P. stuartii.