Fig 1.

(A) Inhibition of bFGF (10 ng/ml)-induced endothelial cell proliferation by 20 nM HKa (open bars) and 60 nM HKa D5 (black bars) is prevented by increasing concentrations of mAb TM-311. Proliferation was measured as described in Materials and Methods. (B) Effect of mAb TM-311 and antibodies against other endothelial cell HK or HKa-binding proteins on the inhibition of endothelial cell proliferation caused by 20 nM HKa. Proliferation was measured in response to 10 ng/ml bFGF. All antibodies were used at a concentration of 300 nM. The control antibody for polyclonal antiurokinase receptor (uPAR) and cytokeratin 1 (CK-1) antibodies was normal rabbit IgG (NRIgG), and that for mAb TM-311 and mAbs against the receptor for the globular heads of C1q (gC1qR) was MOPC-21.