Fig 4.

Immunoprecipitation of endothelial tropomyosin by mAb TM-311. Cell surface proteins on proliferating and confluent human umbilical vein endothelial cells were labeled with N-hydroxysuccinimide (sulfo)-LC-biotin. Equal amounts of protein from detergent extracts of each culture were immunoprecipitated by using TM-311 or MOPC-21. Immunoprecipitated proteins were separated by using 10% SDS/PAGE, transferred to poly(vinylidene difluoride), and detected by using streptavidin-peroxidase and chemiluminescence.