Fig 6.
(A) Specific binding of HKa to purified tropomyosin. Ninety-six-well plates were coated with tropomyosin and then incubated with increasing concentrations of biotin-HKa in the absence or presence of 10 μM Zn2+. Bound ligand was determined by using streptavidin-peroxidase and turbo-TMB, and the curve was fit by nonlinear regression, yielding a Kd of ≈1.6 nM. (B) Inhibition of the binding of 20 nM biotin-HKa to purified tropomyosin by mAb TM-311. (C) Inhibition of the binding of biotin-HKa (10 nM) to purified tropomyosin by HKa D5. The IC50 for inhibition of HKa binding by HKa D5 was ≈8.1 nM.