Table 3.

Determination of experimental error rate during SAGE process

Summary of error rate in three independent SAGE experiments
Experiment	Total collected SAGE tags	Erroneous tags	Error rate, %
1	1,200	20	1.67
2	1,086	18	1.66
3	1,062	18	1.69
Total	3,348	56	1.67
Distribution of error nucleotides
Wild-type tag A	Detected number	Wild-type tag B	Detected number
GTGCACTGAG	1,638	TACCTGCAGA	1,654
Erroneous tag A		Erroneous tag B
ATGCACTGAG	2	CACCTGCAGA	2
GCGCACTGAG	4	TTCCTGCAGA	2
GTACACTGAG	2	TACTTGCAGA	2
GTGCTCTGAG	2	TACCAGCAGA	2
GTGCAATGAG	2	TACCTACAGA	2
GTGCATTGAG	12	TACCTTCAGA	1
GTGCACCGAG	2	TACCTGTAGA	2
GTGCACTGAA	6	TACCTGCTGA	2
GTGCACTGAC	2	TACCTGCAAA	2
GTGCACTGAT	2	TACCTGCAGG	3