Table 1.
Immunogenicity of selected hTRT HLA A2.1-binding peptides
| Peptide | Sequence | Relative avidity | Immunogenicity |
|---|---|---|---|
| p152 | LLARCALFV | 15 | |
| pY152 | YLARCALFV | 5 | 50, 36, 14, 0, 88, 16, 36, 83 |
| p407 | VLLKTHCLP | 10 | |
| pY407 | YLLKTHCLP | 10 | 0, 0, 0, 0 |
| p555 | ELLRSFFYV | 35 | |
| pY555 | YLLRSFFYV | 3 | 72, 46, 12, 0, 21, 18, 27, 97 |
| p572 | RLFFYRKSV | 30 | |
| pY572 | YLFFYRKSV | 1.9 | 45, 24, 9, 44, 92, 21, 73 |
| p675 | LLGASVLGL | >30 | |
| pY675 | YLGASVLGL | 2 | 17, 12, 0, 0 |
| p724 | RLYEVIASI | >30 | |
| pY724 | YLYEVIASI | 3.5 | 6, 0, 0, 0 |
| p1072 | WLCHQAFLL | >30 | |
| pY1072 | YLCHQAFLL | 4 | 17, 0, 0, 0 |
| p540 | ILAKFLHWL | 2.9 | |
| p865 | RLVDDFLLV | 2.5 |
The denomination of peptides denotes the position of the first residue in the predicted amino acid sequence of hTRT (8).
The relative avidity is measured in arbitrary units as described in Materials and Methods.
Data represent the percent (%) specific lysis by primary CTL cultures generated from individual HHD mice immunized with the indicated analogue peptide. Values refer to % lysis of RMAS-HHD cells pulsed with the counterpart wild-type peptide (10−6 M) minus the % lysis of RMAS-HHD cells pulsed with the HLA A2-binding 58GILGFVFTL66 peptide of Influenza virus matrix antigen used as control at an E/T ratio of 50:1. Nonspecific lysis on target cells pulsed with the irrelevant peptide was between 0 and 13%.
p540 and p865 representing previously described high-affinity hTRT peptide (9) are shown by comparison.