Fig 2.
Enhanced tumorigenicity and invasiveness of P815/PD-L1 cells in the syngeneic DBA/2 mice. (A) P815 (○) or P815/PD-L1 (•) cells were inoculated s.c. at 1 × 106 cells per mouse into DBA/2 (Left) or H-2-shared BALB/c nu-nu (Right) mice, and local tumor growth was monitored. The mean tumor volumes and SE of six mice in each group are indicated. *, P < 0.01 by Student's t test. (B) DBA/2 mice (six mice per group) were inoculated with P815 (○) or P815/PD-L1 (no. 1 • and no. 2 ▴) cells as above and the survival rates were monitored. (C) DBA/2 mice inoculated s.c. with P815/PD-L1 cells were autopsied on day 18. Local s.c. tumors showing invasion across the abdominal wall into the peritoneal cavity (a and b) (×40 and ×400), spleen showing massive invasion of tumor cells (c), and liver with focal metastatic region (d).