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Effects of cysteine mutations on secretion of precursor GILT. COS-7 cells expressing wild-type, C91S, C98S, C200S, C211S, and C222S mutant GILT were starved, metabolically labeled with [35S]methionine, and chased for up to 8 h. Secreted GILT was immunoprecipitated from the supernatants with MAP.IP30, and samples were analyzed by nonreducing SDS/12% PAGE and autoradiography. With the exception of C222S mutation, both dimeric and monomeric precursor forms were secreted by COS-7 cells.
