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SP cell analysis of CD45− skeletal muscle cells from Bcrp1−/− mice. Single cell suspensions from skeletal muscle tissue obtained from wild-type and Bcrp1−/− mice were stained with an anti-CD45 antibody and Hoechst dye. The CD45−, nonhematopoietic cells were gated (Left) and analyzed for SP cells (Right). The proportion of SP cells within the CD45− gate is shown for each sample.
