Fig 3.

Expression pattern of the DMT1 5′ and 3′ end variants in mouse tissues. Total RNA from organs and tissue obtained from a C57BL6 mouse were reverse-transcribed by using random primers. PCR amplifications were performed by using specific primers of the alternative 5′ exon (DMT1-1A vs. DMT1-1B) or the alternative 3′ terminal exon (DMT1-IRE vs. DMT1-non-IRE). D1 refers to the 1st cm of the duodenum, D2 and D3 refer to the 2nd and the 3rd cm respectively. All depicted PCR products have the respective predicted sizes. Analysis of total RNA from mouse brain homogenate revealed the expression of the 1B isoform as well as of the IRE and non-IRE 3′ variants, but no evidence for the expression of the 1A isoform in brain could be obtained (data not shown).