Fig 4.

Iron regulation of 5′ end variants of DMT1 mRNA in human cell lines. Semiquantitative RT-PCR was performed on total RNA from cells treated as in Fig. 1 by using 16 PCR cycles for hβ-actin and 25 cycles for DMT1 1A and 1B. The forward primers used to amplify the two 5′ variants of DMT1 hybridized to the respective exon 1 (A or B) and the reverse primer to exon 3. The PCR products have a size of 292 and 223 bp, respectively. Shown is one representative experiment of three.