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. 2026 Feb 7;18(2):218. doi: 10.3390/v18020218
AacCas12b Alicyclobacillus acidoterrestris Cas12b
aM Attomolar
App Application
ATP Adenosine Triphosphate
BIP Backward Inner Primer
BOP Backward Outer Primer
BrCas12b Brevibacillus sp. Cas12b
Bsu Bacillus subtilis
Cas CRISPR-Associated
CCHF Crimean-Congo Hemorrhagic Fever Virus
cDNA Complementary DNA
CRISPR Clustered Regularly Interspaced Short Palindromic Repeats
crRNA CRISPR RNA
CRISPRET CRISPR-Based Rapid and Efficient Test
DENV Dengue Virus
DETECTR DNA Endonuclease-targeted CRISPR Trans Reporter
DNA Deoxyribonucleic Acid
dsDNA Double-Stranded DNA
EBOV Ebola Virus
ELISA Enzyme-Linked Immunosorbent Assays
ERASE Easy-Readout and Sensitive-Enhanced
EXPAR Exponential Amplification Reaction
FIP Forward Inner Primer
fM Femtomolar
FOP Forward Outer Primer
gRNA Guide RNA
HDA Helicase Dependent Amplification
HOLMES One Hour Low-Cost Multipurpose Highly Efficient System
HPV Human Papillomavirus
HUDSON Heating Unextracted Diagnostic Samples to Obliterate Nucleases
LAMP Loop-Mediated Isothermal Amplification
LASV Lassa Virus
Lift-CM Lift-Heater Centrifugal Microfluidic Platform
Loop B Loop Backward
Loop F Loop Forward
MARV Marburg virus
mLAMP Multiplexing LAMP
MRSA Methicillin-Resistant Staphylococcus aureus
NASBA Nucleic Acid Sequence-Based Amplification
PAM Protospacer Adjacent Motif
PCR Polymerase Chain Reaction
POC Point of Care
POCT POC Test
RAA Recombinase Aided Amplification
REASSURED Real-time Connectivity, Ease of Specimen Collection, Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-Free and Environmentally Friendly, and Deliverable to End-Users
RNA Ribonucleic Acid
RPA Recombinase Polymerase Amplification
RT-LAMP Reverse-Transcription LAMP
RT-qPCR Quantitative Reverse-Transcription PCR
RT-RAA Reverse-Transcription RAA
RT-RPA Reverse-Transcription RPA
RVFV Rift Valley Fever Virus
SARS-CoV-2 Severe Acute Respiratory Syndrome Coronavirus-2
SDA Strand Displacement Amplification
SEC-disc Spatially Encoded Centrifugal Disc
SHERLOCK Specific High-Sensitivity Enzymatic Reporter UnLOCKing
SNP Single Nucleotide Polymorphisms
ssDNA Single-Stranded DNA
STOP SHERLOCK Testing in One-Pot
TCEP-EDTA Tris(2-carboxyethyl)phosphine hydrochloride–Ethylenediaminetetraacetic Acid
tracrRNA trans-Activating crRNA
VHF Viral Hemorrhagic Fever
WHO World Health Organization
ZIKV Zika Virus