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. 2002 Sep 6;99(19):12363–12368. doi: 10.1073/pnas.192445199

Fig 2.

Fig 2.

Effects of NAC against ribose-induced decreases in glucose-induced insulin secretion, content, and mRNA and abrogation of these preventive effects of NAC by BSO in HIT-T15 cells (A–C) and rat islets (D–F). HIT-T15 cells were precultured in 30 mM ribose with or without 10 mM NAC with or without 50 μM BSO for 72 h. (A) Insulin secretion from cells incubated in KRB buffer containing increasing concentration of glucose for 2 h (*, P < 0.01 vs. control; repeated measurement ANOVA). (B) Intracellular insulin content (*, P < 0.05; one-way ANOVA). (C) Insulin mRNA level (*, P < 0.01, and †, P < 0.001; one-way ANOVA). Rat islets were precultured in 50 mM ribose with or without 10 mM NAC or 100 μM BSO for 72 h. (D) Insulin secretion from islets incubated in KRB buffer containing 5.6 or 16.7 mM glucose for 1 h (*, P < 0.001, and †, P < 0.001; one-way ANOVA). (E) Intracellular insulin content (*, P < 0.05, one-way ANOVA). (F) Insulin mRNA level (*, P < 0.05, one-way ANOVA). All data are presented as means ± SE for three or four independent experiments with duplicate or triplicate determinations.