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. 2002 Sep 6;99(19):12363–12368. doi: 10.1073/pnas.192445199

Fig 5.

Fig 5.

Effects of adenovirally (Ad) induced GPx overexpression in Wistar rat islets against ribose-induced decreases in insulin mRNA, content, and secretion. (A) GPx activity 72 h after infection (*, P < 0.001, one-way ANOVA). (B) Effects of GPx overexpression on ribose-dependent decreases on insulin mRNA levels normalized to mRNA levels in uninfected control islets (*, P < 0.001, one-way ANOVA). Islets were incubated in KRB buffer containing 5.6 or 16.7 mM glucose for 1 h to measure insulin secretion before extraction of insulin content. (C) Intracellular insulin content was decreased significantly by 72-h exposure to ribose in both the uninfected and control adenoviral-infected controls (†, P < 0.05, one-way ANOVA). Overexpression with the GPx adenovirus partially prevented the ribose-induced decrease in insulin content (P = not significant from control, one-way ANOVA). (D) Similar results were observed with glucose-induced insulin secretion with islets treated as described for C. All data are presented as means ± SE for 5–8 independent experiments with duplicate or triplicate determinations.