3AGJ	Scaffolded MPER antigen based on PDB 3AGJ
3DA	DADADA N-terminal motif
AEAAAK	Rigid α-helical linker motif AEAAAK (EAAAK repeats)
AIDA	Adhesin Involved in Diffuse Adherence
AIDA-I	Adhesin Involved in Diffuse Adherence I autotransporter
AQQASSS	Semi-flexible α-helical linker motif AQQASSS
AT	Autotransporter
ATs	Autotransporters
AUC	Area Under the Curve
AUC(ELISA)	ELISA area under the curve
AUC(FLOW)	Flow cytometry area under the curve
BCR	B-cell receptor
BSA	Bovine serum albumin
BSL-2	Biosafety level 2
CFU	Colony-forming unit
DBTL	Design–Build–Test–Learn
EC50	Half-maximal effective concentration
ELISA	Enzyme-Linked Immunosorbent Assay
Env	Envelope glycoprotein
FBS	Fetal bovine serum
FDR	False discovery rate
FLIC	Flagellin fragment (TLR5 agonist)
FP	Fusion Peptide
FP-like	Fusion peptide-like
GC	Germinal center
HBSS	Hank’s Balanced Salt Solution
HET3	Four-way crossbred HET3 mouse stock
HIV	Human Immunodeficiency Virus
HIV-1	Human Immunodeficiency Virus type 1
HRP	Horseradish peroxidase
ID50	50% inhibitory dilution titer
ID80	80% inhibitory dilution titer
IgG	Immunoglobulin G
KWC/GRB	Killed Whole-Cell Genome-Reduced Bacteria
LB	Luria–Bertani broth/agar
ME5125	Genome-reduced Escherichia coli strain ME5125
MG1655	Escherichia coli strain MG1655
MPER	Membrane-Proximal External Region
NCI	National Cancer Institute
NIAID	National Institute of Allergy and Infectious Diseases
NIFA	National Institute of Food and Agriculture
NIH	National Institutes of Health
OD600	Optical density at 600 nm
OX40L	OX40 ligand
PADRE	Pan DR Epitope (universal CD4+ T-helper epitope)
PBS	Phosphate-buffered saline
PC1	Principal component 1
PC2	Principal component 2
PCA	Principal component analysis
PEDV	Porcine epidemic diarrhea virus
RAU	Relative antibody units
RLU	Relative luminescence units
ROC	Receiver operating characteristic
SEM	Standard error of the mean
SOC	Super Optimal broth with Catabolite repression
SPF	Specific-pathogen-free
TBS	Tris-buffered saline
TBST	Tris-buffered saline with Tween-20
TLR2	Toll-like receptor 2
TLR2/4	Toll-like receptors 2 and 4
TLR4	Toll-like receptor 4
TLR5	Toll-like receptor 5
TMB	Tetramethylbenzidine
TZM-bl	HIV-1 reporter cell line TZM-bl
USA	United States of America
USDA	United States Department of Agriculture
UVA	University of Virginia
V1	Scaffold-MPER vaccine 1
V2	Scaffold-MPER vaccine 2
V3	Scaffold-MPER vaccine 3
V4	Scaffold-MPER vaccine 4
V5	Scaffold-MPER vaccine 5
bnAb	Broadly neutralizing antibody
bnAbs	Broadly neutralizing antibodies
gp41	Glycoprotein 41
rSIP	Recombinant surface immunogenic protein