Fig 2.

Dysfunction of bvrR and bvrS alters the profile of B. abortus group 3 Omps. 2DGE (A and B) and the corresponding Western blot analysis (C and D) of outer membrane fragments of B. abortus 2308 wild type and BvrS mutant. The arrows indicate the silver-stained Omp3a and Omp3b spot groups. Numbers indicate spots immunodetected with anti-Omp25 mAbs. Notice that spots marked as Omp3a in A are the same as spots 1, 2, and 3 in C, and that spot “a” is detected in the wild type but only barely in the mutant. On the contrary, spots α, β, and γ present in the mutant were not detected in the wild type. Trace amounts of spot 9 were detected in the mutant.