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. 2026 Jan 23;302(3):111199. doi: 10.1016/j.jbc.2026.111199

Figure 2.

Figure 2

Activities of engineered RINGdomains and xRNF38 in xUB transfer.A, self-ubiquitination of phage-selected RING mutants LZ1-4 in reactions with biotin-wtUB, xUba1(UFD), and xUbcH5b following the conditions of phage selection. B, activities of RING mutants LZ1-4 in self-ubiquitination reactions with xUB transferred from the xUba1-xUbcH5b pair. C, the activity of full-length xRNF38 with engineered mutations from LZ1 in the self-ubiquitination reaction with xUB and the xUba1-xUbcH5b pair. D, xRNF38-catalyzed p53 ubiquitination reaction with xUB and the xUba1-xUbcH5b pair.