Fig 4.

Molecular masses of denatured and native LeGCHI. (A) Immunoblot of proteins extracted from yeast strain 971/6a transformed with pVT103-U alone (V) or containing LeGCHI (L). Lanes contained 25 μg of protein; the positions of molecular mass markers (kDa) are shown. (B) Elution profile of recombinant LeGCHI activity from a Superdex 200 HR 10/30 column loaded with 0.25 mg of protein extracted from strain 971/6a expressing LeGCHI. The column calibration is shown (Inset). The molecular mass of GCHI was determined to be 91 ± 5 kDa (mean ± SE of four experiments). Standards were carbonic anhydrase (29 kDa), BSA (66 kDa), β-amylase (200 kDa), apoferritin (443 kDa), and thyroglobulin (669 kDa).