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. 2002 Sep 9;99(19):12489–12494. doi: 10.1073/pnas.192278499

Fig 5.

Fig 5.

Developmental changes in levels of LeGCHI mRNA, LeGCHI protein, and folate in tomato fruits. (A) Northern analysis of LeGCHI mRNA. Lanes contained 10 μg of total RNA from fruits at mature green (MG), breaker (Br), ripe (R), and red-ripe (RR) stages or from young leaves. Blots were probed with the LeGCHI coding sequence (Upper) and then with an 18S ribosomal DNA sequence (Lower) as a loading control. The sizes (kb) of the hybridizing RNAs are indicated. (B) Western analysis of LeGCHI protein in fruits. Lanes contained 20 μg of protein; the positions of molecular mass markers (kDa) are shown. The Upper blot was probed with anti-GCHI serum and the Lower blot with control antiserum depleted in anti-GCHI antibodies by preincubation with purified recombinant LeGCHI. (C) 5-Methyltetrahydrofolate (methyl-THF) levels in fruits. The data are means of three replicates ± SE. FW, fresh weight.