Figure 1.

Effects of dietary Na⁺ and a Liddle’s mutation on γENaC processing. A. Western blots of mouse kidney microsomes from mice on low- Na⁺, control- Na⁺ and high- Na⁺ diets. Each lane from a single blot was loaded with equal amounts of protein (low Na 80 μg; control Na 40 μg; high Na 50 μg) from individual WT or Liddle mouse kidneys. Sexes of the mice are marked on each lane (m = male, f = female) B. Band densities for full-length and cleaved forms were normalized to those of the cleaved form of WT animals. The ratios of cleaved/full-length are plotted as absolute values. Data are shown as means ± SEM for n = 8 (low-Na), or 4 (control Na, high Na). p-values from Student t-tests are shown above the bars.