Figure 3.

Effect of aldosterone infusion on γENaC processing. Mice on control diet were implanted with osmotic minipumps infusing aldosterone at different rates. A. Western blot of kidney microsomes from individual animals infused with 0, 0.24, 0.8, 2.4 or 7 μg/day. Each lane was loaded with 60 μg total protein. B. Band density for cleaved γENaC as a function of the aldosterone infusion rate. Data were normalized to values at infusion rates of 2.4 μg/day and fit with a hyperbolic function using the equation $\mathrm{A}\left(\mathrm{r}\right)={\mathrm{A}}_0+(\left({\mathrm{A}}_{\mathrm{m}\mathrm{a}\mathrm{x}}–{\mathrm{A}}_0\right)/\left(1+{\mathrm{r}}_{0.5}/\mathrm{r}\right)$ where ${\mathrm{A}}_0=0.39,{\mathrm{A}}_{\mathrm{m}\mathrm{a}\mathrm{x}}=1.07$ and ${\mathrm{r}}_{0.5}=0.8\mathrm{\mu }\mathrm{g}/\mathrm{d}\mathrm{a}\mathrm{y})$ C. Plasma aldosterone as a function of infusion rate. At low rates the relationship was approximately linear with ${\mathrm{P}}_{\text{aldo}}=0.66+0.34•\mathrm{r}$. All mice were males.