Figure 4.

Effects of aldosterone infusion on WT and Liddle mice. Animals were implanted with osmotic minipumps to infuse aldosterone at rates of either 0.3 (A) or 48 μg/day (B). Each lane was loaded with 50 μg (A) or 80 μg (B) protein from an individual animal. The sex of individual animals is marked on each lane (m = male, f = female). Band densities were normalized to those of the WT cleaved form. Males and females were normalized separately. The ratios of cleaved/full-length are plotted as absolute values. Bar graphs plot mean ± SEM for n = 4 pairs. (A) and n = 10 pairs (B). p-values from Student t-tests are shown above the bars.